| Penulis/Author |
Anwar Rovik (1); dr. Marselinus Edwin Widyanto Daniwijaya, Ph.D., Sp. MK (2); Endah Supriyati (3); Ayu Rahayu, S.Si., M.Si. (4); Dian Aruni Kumalawati (5); Utari Saraswati (6); dr. Anastasia Evi Handayaningsih, Ph.D (7); MIFTA PRATIWI RACHMAN (8); Dr.rer.nat. Risky Oktriani, S.Si., M.Biotech., M.Sc. (9); IRIANTI KURNIASARI (10); DAMIANA SAPTA CANDRASARI (11); Indah Nurhayati (12); RIZKI SHOLEH (13); BUDI ARIANTO (14); Warsito Tantowijoyo (15); dr. Riris Andono Ahmad, MPH, Ph.D (16); Prof. dr. Adi Utarini, M.Sc., MPH.,Ph.D. (17); Prof. dr. Eggi Arguni, M.Sc., Ph.D., Sp.A(K). (18) |
| Abstrak/Abstract |
Wolbachia naturally presents in a large number of insects and other arthropod species. The Wolbachia strain wMel from Drosophila melanogaster has been stably transinfected into Aedes aegypti where it stops the mosquito host from being infected with medically important arbovirus like dengue. Consequently, Ae. aegypti infected with wMel have been released in Indonesia as a public health intervention against dengue. This study genetically compared wMel from Yogya field-caught D. melanogaster and the wMel in stably transfected Ae. aegypti used for field releases in Yogyakarta, Indonesia. The genetic similarity between wMel Wolbachia was evaluated by sequencing of Wolbachia surface protein (wsp) gene and some polymorphic genomic regions of insertion sites (IS) and variable number tandem repeats (VNTR) loci. The sequence of the Wolbachia surface protein (wsp) gene was 100% identical between hosts. There is no insertion sequence among specimens. The insertion sequence IS-WD1310 was identical between wMel from both hosts and among other strains, as well as the IS-WD516/7. The VNTR-141 period was identical within wMel from both hosts and among other strains, the VNTR-105 as well. Wolbachia Yogya field-caught D. melanogaster and Wolbachia strain wMel present in Ae. aegypti used for bio-control of dengue were genetically identical. These findings provide beneficial understanding to answer the public attention on safety issues, especially on the genetic similarity between Wolbachia strain in the natural and transfected hosts of this novel technology for dengue control. |
