VALIDATION OF UV-VIS SPECTROPHOTOMETRIC METHOD TO DETERMINE DRUG RELEASE OF QUERCETIN LOADED-NANOEMULSION
Penulis/Author
VANIA SANTIKA PUTRI (1); Dr.rer.nat. Ronny Martien, M.Si. (2); Dr. Adhyatmika, M.Biotech., Apt. (3); apt. Arif Nur Ikhsan, M.Pharm.Sci. (4)
Tanggal/Date
30 2022
Kata Kunci/Keyword
Abstrak/Abstract
Oral bioavailability of quercetin has been limited due to its poor water solubility. The
formulation of quercetin into a spontaneously formed nanoemulsion system can increase its
oral bioavailability. Quantification of the released quercetin from the nanoemulsion matrix is
one of the critical parameters that needs adjustment, development and validation of current
analytical methods. A simple validated UV/Vis spectrophotometric method to analyze
quercetin released from the nanoemulsion matrix has been developed to study the drug
release profile of quercetin loaded-nanoemulsion. Quercetin analysis was carried out at a
maximum wavelength of 254 nm. The method showed linearity with a correlation coefficient
(r) value of 0.9998 in the range of 4 - 12 μg/mL. The results also demonstrated that the
procedure is accurate and precise, with recovery (%) in the range of 99.65 - 100.1312 % and
RSD (%) as ≤ 2%. The limit of detection (LOD) and limit of quantitation (LOQ) values were
found to be 4.0535 μg/mL and 13.5118 μg/mL, respectively. The developed method was
found to be valid to analyze quercetin released in nanoemulsion preparations.
Rumpun Ilmu
Farmasetika dan Teknologi Farmasi
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Full dokumen.pdf
[PAK] Full Dokumen
2
Validation of UV-Vis Spectrophotometric Method to Determine Drug Release of Quercetin Loaded-Nanoemulsion-2.pdf
