| Abstrak/Abstract |
Pentagamavunon-0 has a potent anti-inflammatory activity but has poor solubility
and absorption properties, which can be improved by SNEDDS formulation. One of
the most critical parameters of the optimization of SNEDDS is the solubility of PGV-0 in various oils, surfactants, co-surfactant, and in the final product. This study is
aimed to validate a simple and inexpensive UV-Vis spectrophotometric method for
PGV-0 quantification in SNEDDS formulation. The assay was performed using UVVis spectrophotometer, methanol as the solvent, and Vis detection at wavelength
of 419 nm. The linearity, limit of detection (LOD), limit of quantity (LOQ),
precision, and accuracy of the method was determined. The solubility study was
performed by modified shake flask method, using labrasol as a vehicle model. The
insoluble PGV-0 was separated and cleaned from the filtrate, quantified by validated
UV-Vis spectrophotometry, and the solubility of PGV-0 was determined.
Validation measurement was carried out in the range of 1-8 ppm. The results
showed a good linearity with r=0,99949, LOD=0.29 ppm, and LOQ=0.95 ppm. The
accuracy expressed as recoveries were found to be 98.32-100.74%. The precision
was good (RSD=1.29–1.93%). The precision was good (RSD=1.29–1.93%). |
