UV‐Vis Spectrophotometry and UPLC–PDA Combined with Multivariate Calibration for Kappaphycus alvarezii (Doty) Doty ex Silva Standardization Based on Phenolic Compounds
Penulis/Author
SELMA MUTIARAHMA (1); VENANSIUS GALIH P P (2); WENI CHANIAGO (3); Ceferino Carrera (4); Prof. Dr. Ir. Sri Anggrahini, M.S. (5); Miguel Palma (6); Dr. Widiastuti Setyaningsih, S.T.P., M.Sc. (7)
Tanggal/Date
27 2021
Kata Kunci/Keyword
Abstrak/Abstract
The algae Kappaphycus alvarezii is considered an important raw material for industrial practices, producing high economic value of various derived products. However, the quality of this commodity, which can be indicated by the level of phenolic compounds, may vary due to growth factors, including cultivation sites. An analytical UV‐Vis spectrophotometry method coupled with chemometrics was proposed to standardize the red alga based on the content of phenolic com pounds. The correlation between the UV‐Vis spectra and UPLC–PDA results, combined with a multivariate calibration of the K. alvarezii extracts, was analyzed. The extracts were prepared using an ultrasound‐based technique and subsequently subjected to UV‐Vis spectral measurements at 200–800 nm and UPLC–PDA at 260 and 330 nm. Chemometric techniques and partial least squares (PLS) were applied to the acquired data to build a reliable analysis of the phenolics in the K. alvarezii extracts. The result showed that the wavelength combination of 200 450 and 600–690 nm provided a valid method for quantitative analysis of the studied phenolics that belong to hydroxybenzoic acid, hydroxycinnamic acid, and flavonoid with a coefficient of regression (R2) > 0.96 in the calibra‐ tion and validation models, along with an RMSEC and RMSEP value < 8%. The method was then employed to characterize the K. alvarezii samples from 13 different cultivation areas. Principal com‐ ponent analysis (PCA) generated principal components that produced a clear distribution among the samples of K. alvarezii based on phenolic compounds corresponding to the geographical origin.
Rumpun Ilmu
Teknologi Hasil Pertanian
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
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Revisi_bukti korespondensi mbak selma.pdf
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UV?Vis Spectrophotometry and UPLC–PDA Combined with Multivariate Calibration for Kappaphycus alvarezii (Doty) Doty ex Silva Standardization Based on Phenolic Compounds (4).pdf
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Scipharm (UV Vis Spectrophotometry and UPLC PDA Combined with Multivariate Calibration__) Full Dokumen.pdf
