| Penulis/Author |
HANA WIDIAWATI (1); Sukirno, S.Si., M.Sc., Ph.D. (2); Dr. Siti Sumarmi (3); Drs. Hari Purwanto, M.P., Ph.D. (4); Dr. R.C. Hidayat Soesilohadi, M.S. (5); Drs. Ign. Sudaryadi, M.Kes. (6) |
| Abstrak/Abstract |
Spodoptera exigua (Lepidoptera: Noctuidae) is the common pest known for attacking shallot crop. Baculovirus
(Nucleopolyhedrovirus : NPV) is a biological agent that is widely used as the pest control agent. However, the activity
of NPV is deteriorated when applied in the field due to the influence of ultraviolet from the sun. Attacus atlas
silkworm cocoon has UV protectant ability due to the presence of sericin protein. Thus, the aim of this study is to
determine the degree of UV protectant ability from A. atlas cocoon extract for NPV to prevent its deterioration and to
increase its effectiveness against S. exigua pest. The stages of this study consist of UVB irradiation of A. atlas cocoon
extract as NPV protectant and pathogenicity test of NPV activity against the first instar larvae of S. exigua.
Experiments were carried out by exposing NPV to UVB irradiation for 0, 1, 2, 3, 4 weeks long with the addition of A.
atlas cocoon extract in varied concentrations (0, 0.5, 1, 2, and 2.5%). The concentrations derived from 7.5 gram A.
atlas cocoon was dilluted 1 gram TRO and 150 ml aquades. The results proved the UV protectant ability of A. atlas
cocoon for NPV. After a week of UVB irradiation, the NPV applied in A. atlas cocoon extract (0-2.5%) caused larval
mortality of 43.33%, 76.67%, 60%, 65%, 80%, respectively. After 2 weeks of UVB irradiation, the NPV applied in A.
atlas cocoon extract (0-2.5%) caused larval mortality of 46.67%, 78.33%, 71.67%, 66.67%, 66.67%, respectively.
Thus, for further applications, the findings of this research need to be tested in an actual field condition. |
