| Abstrak/Abstract |
The objective of this study was the evaluation of fungal solid-state fermentation (SSF) for
the production of alginate lyase and extraction of uronic acids from Sargassum sp. For this
purpose, the fungi Trichoderma asperellum, Aspergillus oryzae, and Rhizopus oryzae were ap
plied (alone or combined)toSargassumsp.biomassthroughSSF(107sporesgbiomass−1, 30°C,
and 7 days of treatment). In general, individual SSF with all three fungi degraded the
biomass, achieving a marked synergy in the production of cellulase, laminarinase, and
alginate lyase activities (especially for the last one). Trichoderma was the most efficient spe
cies in producing laminarinase, whereas Rhizophus was the best option for producing al
ginate lyase. However, when dual combinations were tested, the maximal values of algi
nate lyase activities were reached (13.4 ± 0.2 IU gbiomass−1 for Aspergillus oryzae and Rhizopus
oryzae). Remarkably, uronic acids were the main monomeric units from algal biomass sol
ubilization, achieving a maximum yield of 14.4 mguronic gbiomass−1, with the A + R condition
being a feasible, eco-friendly alternative to chemical extraction of this monomer. Addi
tionally, the application of all the fungal pretreatments drastically decreased the total phe
nolic content (TPC) in the biomass from 369 mg L−1 to values around 44–84 mg L−1, mini
mizing the inhibition for possible subsequent biological processes in which the residual
solid can be used. |
