| Abstrak/Abstract |
This research aimed to iden?fy the heterozygosity of an F2 population of a black rice and white rice cross using microsatellite markers. Rice was grown and harvested in a field approximately 500 m2 in size in Pakem (Sleman, Yogyakarta, Indonesia), and analyzed in the Genetics and Plant Breeding Laboratory of the Faculty of Agriculture, Universitas Gadjah Mada. The research materials consisted of an F2 S × G population composed of a cross of black rice (S) and white rice
Situbagendit (G), female parent of black rice (S), male parent of white rice (G), chemical and organic fertilizer, chemicals and tools for molecular activity and three microsatellite markers related to color properties (RM 220, RM 224, and RM 252). All of the plant populations (generation F2, parent female, parent male) were planted in the field until their harvest. Young leaves (30 days in age after planting) were molecularly analyzed using three microsatellite markers (RM 220, RM 224, and RM 252). This encompassed DNA isolation, PCR reaction, and the visualization of the PCR results using Metaphore Agarose Gel Electrophoresis. The results showed that the percentage of the number of individual plants showing a heterozygous pattern in the F2 S × G population was 50% (RM 220), 40% (RM 224), and 60% (RM 252). As such, the RM 252 microsatellite marker
can be used for marker-assisted selection in the crossbreeding of black rice |
