Upregulation of p16, Bax and Bcl-2 mRNA expression associated with epithelial apoptosis and myofibroblast proliferation in kidney fibrosis model in mice
Penulis/Author
IKE SULISTIYOWATI (1); dr. Junaedy Yunus, M.Sc., Ph.D. (2); Prof. Dr. dr. Dwi Cahyani Ratna Sari, M.Kes.PA(K) (3); dr. Nur Arfian, Ph.D. (4)
Tanggal/Date
30 2020
Kata Kunci/Keyword
Abstrak/Abstract
Background: Cellular senescence may play a role in the development of kidney fibrosis,
but its specific association with apoptosis or proliferation have yet to be determined.
Objectives: This study aims to determine the effects of unilateral ureteral obstruction
(UUO) on proliferation, cellular senescence and apoptosis in kidney fibrosis.
Methods: A unilateral ureteral obstruction (UUO) procedure was performed to induce
kidney fibrosis in 24 Swiss mice (3 months old, 30 g–40 g). Mice were sacrificed on day 3 (UUO3,
n = 6), day 7 (UUO7, n = 6) and day 14 (UUO14, n = 6). Sham operation (SO) procedures were
performed on the control group. The expression of Bcl-2, p16 and Bax mRNA was quantified with
reverse transcription polymerase chain reaction (RT-PCR). Immunohistochemical (IHC) staining
with anti-Bcl-2 and p53 antibodies was used to determine the localisation of proliferation and
apoptosis. Data were analysed using one-way ANOVA followed by a post hoc least significant
difference (LSD) test (p< 0.05)
Results: RT-PCR analysis showed higher mRNA expression of Bcl-2, p16 and Bax in
the UUO groups compared with SO group (p<0.05). Immunostaining showed that Bcl-2 and
p53 expression in tubular epithelium in the UUO groups, except Bcl-2 expression was found in
interstitial areas of UUO14 group.
Conclusion: Senescence in UUO might be associated with epithelial apoptosis and
myofibroblast proliferation.
Rumpun Ilmu
Ilmu Kedokteran Dasar & Biomedis
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
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Upregulation of p16 Bax and Bcl-2 mRNA Expression Associated with Epithelial Apoptosis and Myofibroblast Proliferation in Kidney Fibrosis Model in Mice.pdf
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Upregulation of p16, bax and bcl-2 mrna expression associated with epithelial apoptosis and myofibroblast proliferation in kidney fibrosis model in mice.pdf
