| Penulis/Author |
dr. Nungki Anggorowati, Sp.PA(K)., Ph.D. (1); Dr. dr. Ahmad Ghozali, SpPA(K) (2); Prof. Dr. dr. Irianiwati, Sp.PA.(K) (3); Prof. Dr. dr. Dwi Cahyani Ratna Sari, M.Kes.PA(K) (4); Dr. dr. Muhammad Mansyur Romi, S.U., PA(K) (5); dr. Nur Arfian, Ph.D. (6) |
| Abstrak/Abstract |
Background Ovarian carcinoma leads to mortality from gynecologic malignancy as a result of prompt metastatic behavior. Heparanase and endothelin-1/endothelinA receptor (ET-1/ETAR) axis play role in ovarian carcinoma pathology. This study is to elucidate correlation between both systems in ovarian carcinoma. Methods Fresh tissues and paraffin sections were collected from 30 patients with ovarian carcinoma with 4 variants. RT-PCR was done for quantification ppET-1, ETAR, and heparanase expression. Histoscore of ETAR and heparanase as well as Ki67 quantification were done based on IHC staining. Results The age was ranged from 15 to 71 y.o. (median age 49.5 y.o.). The heparanase and ETAR histoscore, prepro endothelin-1 (ppET-1), and ETAR mRNA level, as well as Ki-67, were significantly higher in ovarian carcinoma than in benign or borderline groups, regardless histopathological type. Heparanaseimmunohistochemicalhistoscore was correlated with ETAR histoscore and ETAR mRNA level (r=0.551, p=0.003). The level of ppET-1 mRNA was correlated with both ETAR mRNA level and ETAR histoscore (r=0.603, p=0.001 and r=0.455, p=028, respectively). The ovarian neoplasm with high ppET-1 mRNA level tended to have high heparanase mRNA level, yet only weak correlation was perceived between ppET-1 and heparanase mRNA level (r=0.354, p=0.07). Ki-67 was correlated with heparanase and ETAR histoscore (r=0.381, p=0.038; r=0.477, p=0.008, respectively). Conclusions The correlation of heparanase and ETAR in the current study marks possible crosstalk between ET-1/ETAR axis and heparanase. Furthermore it may enhance anti-tumor and anti-metastatic activities of ETAR and heparanase inhibitors. |
