| Abstrak/Abstract |
Abstract: 3,4-dihydro-6-[4-{3,4-dimethoxybenzoyl}-1-piperazinyl]-2(1H)-quinolinone-vesna rinone is a new and novel inotropic drug
that has unique and complex mechanisms of action such as unique anti-proliferating, differentiation-inducing and apoptosis-inducing
drug against several human malignancies. The aim of study was to evaluate the up-regulation of p27Kip1 and caspase-9 expression in
oral malignant Burkitt’s lymphoma (Raji) cell induced by vesnarinone. Study on chemotactic migration and apoptosis cell. In the
present study, laboratories pure experimental with post-test only control group design was confirmed. Raji cells were incubated with
vesnarinone doses of 0, 6.25x 10-3, 1.25x10-2, 2.5x10-2 and 5x10-2 M. After 24 h incubation, chemotactic cell migration was examed by
Boyden chamber kit 48 well. Cell apoptosis activity was observed with caspase-9 colorimetric assay. Furthermore, to evaluate the level of
p27Kip1 protein was detected by Western blot assay. Data were analyzed by Anova one way followed by post-hoc LSD test for significance
level of 95%. Results revealed vesnarinone was markedly suppressed the activity of chemotactic migration and significantly induced
apoptosis of Raji cell characterized by up-regulation of p27Kip1 protein and proteolytic activity caspase-9. In conclusion, vesnarinone
increased up-regulation of p27Kip1and caspase-9 in oral malignant Burkitt’s lymphoma cell chemotactic migration suppression and
apoptosis induction targeting this molecule could represent a promising new therapeutic approach to this type of tumor. |
