Ultraviolet-B Irradiation Induces Resistance against Powdery Mildew in Cucumber (Cucumis sativus L.) through a Different Mechanism Than That of Heat Shock-Induced Resistance
Penulis/Author
Dinar Mindrati Fardhani (1); Agung Dian Kharisma (2); Tomoyuki Kobayashi (3); Nur Akbar Arofatullah, S.P., M.Biotech., Ph.D. (4); Makoto Yamada (5); Sayuri Tanabata (6); Yumi Yokoda (7); Prof. Ani Widiastuti, S.P., M.P., Ph.D. (8); Tatsuo Sato (9)
Tanggal/Date
29 2022
Kata Kunci/Keyword
Abstrak/Abstract
Heat shock treatment (HST) and UV-B irradiation can reduce pathogen infection in crops. However, information on the mechanism of UV-B action is limited. Here, we investigated the mechanism of UV-B-induced resistance against powdery mildew in cucumber and compared it to that of heat-shock-induced resistance. We measured the percentage of leaf area showing disease symptoms and examined the expression levels of defense- and heat-shock-related genes across treatment groups. UV-B irradiation (intensity, 5 µW/cm2) for 4 h/d followed by pathogen inoculation reduced the appearance of powdery mildew by 21.17% compared with the control group. Unlike HST—which induces systemic resistance—UV-B irradiation induced local resistance in cucumber, as indicated by local changes in gene [removed]Chi2 and ETR2). UV-B-treated plants inoculated with powdery mildew showed higher expression levels of Chi2, ETR2, and LOX6 than plants that were either treated with UV-B or inoculated. UV-B had no major effects on systemic acquired resistance or heat shock transcription factors, which are known to be affected by HST. Combined HST and UV-B had a strong synergistic effect in reducing powdery mildew in cucumber. Our results indicate that UV-B treatment likely operates through a different mechanism than HST in triggering cucumber resistance against powdery mildew infection.
Rumpun Ilmu
Bioteknologi Pertanian dan Perkebunan
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
agronomy-12-03011-v2.pdf
[PAK] Full Dokumen
2
Turnitin-Ultraviolet-B Irradiation Induces Resistance against Powdery Mildew (1).pdf
