Karya
Judul/Title Tylophorine Abrogates G2/M Arrest Induced by Doxorubicine and Promotes Increased Apoptosis in T47D Breast Cancer Cells
Penulis/Author NOFRAN PUTRA PRATAMA (1); Septi Wulandari (2); Prof. Dr. apt. Agung Endro Nugroho, S.Si., M.Si. (3); Prof. Dr.rer.nat. Nanang Fakhrudin, M.Si., Apt. (4) ; Prof. Dr. Puji Astuti, S.Si., M.Sc., Apt. (5); Prof. Dr. Sudarsono, Apt. (6)
Tanggal/Date 2018
Kata Kunci/Keyword
Abstrak/Abstract Background: The effects of tylophorine, a natural alkaloid found in Tylophora indica, administered as a single compound or in combination with doxorubicin on cell cycling and apoptosis were assessed in T47D breast cancer cells, selected as a model system for breast cancer. Methods: Cell cycle distribution and apoptosis were examined by ?ow cytometry. Caspase 3 and 9 expression was determined by immunocytochemistry.Result: We found that tylophorine did not signifcantly in?uence the cell cycle distribution of T47D cells. However, the alkaloid did prevent accumulation of cells in the G2/M phase. In addition, tylophorine increased the number of apoptotic cells. Expression of proapoptotic proteins (caspases 3 and 9) was up-regulated upon administration of tyloporine alone or in combination with doxorubicin. Conclusions: Tylophorine alone or in combination with doxorubicin induced apoptosis in T47D breast cancer cells through modulation of the cell cycle and affecting the expression of caspases 3 and 9.
Rumpun Ilmu Biologi Farmasi
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
118_Asian Pacific Journal of Cancer Prevention_tambah.pdf[PAK] Bukti Korespondensi Penulis
2Tylophorine Abrogates G2_M Arrest Induced by Doxorubicine and Promotes Increased Apoptosis in T47D Breast Cancer Cells.pdf[PAK] Cek Similarity
3Tylophorine Abrogates G2M Arrest Induced by Doxorubicine.pdf[PAK] Full Dokumen
4PEER REVIEW_PUJI ASTUTI 12_.pdf[PAK] Peer Review
5Scopus - Asian Pacific Journal of Cancer Prevention _ Signed in.pdfDokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)