| Abstrak/Abstract |
Trypanosomiasis or surra is a protozoa disease, caused by Trypanosoma evansi. It affects a large number of wild and domesticated animals in Indonesia, especially cattle, buffalo, and horse. Several species of hematophagous flies, including tabanids and Stomoxys, are implicated in transferring infection from host to host, acting as mechanical vectors. The aim of this research was to explore the vectors, flies identification, and Trypanosoma detection. Fly samples were collected from Central Java and Yogyakarta by using NZ-1 trap to study their presence, abundance, seasonal variation, and species diversity. Trypanosomes were collected from the cattle in those areas during the months of May 2016 to September 2016. The trap installations were done in the morning and finished in the afternoon; observations were conducted in the morning at 8 a.m., in the noon at 12 p.m., and in the afternoon at 6 p.m. Types of flies caught in Yogyakarta and Brebes, Central Java, were Tabanus rubidus (16.9% and 22.5%), Tabanus striatus (5.4% and 3.6%), Stomoxys calcitrans (51.2% and 42.3%), Haematobia irritans (19.6% and 26.1%), and Hippobosca sp. (6.9% and 5.4%). By using primer ESAG 6/7 in the PCR detection, it detected 450 bp bands, and ITS-1 primer showed 237 bp bands. Many flies were caught in the morning and afternoon, since it was quite hot during August to October (dry season). These results can be used as a baseline data and surra control program as well. |
