Total flavonoid contents and in silico study of flavonoid compounds from Meniran (Phyllanthus niruri L.) towards alpha-amylase and alpha-glucosidase enzyme
Penulis/Author
Sri Luliana (1); Rise Desnita (2); Dr.rer.nat. Ronny Martien, M.Si. (3); Prof. Dr. apt. Arief Nurrochmad, M.Si., M.Sc. (4)
Tanggal/Date
2019
Kata Kunci/Keyword
Abstrak/Abstract
Meniran (Phyllanthus niruri L.) is a plant that is reported to contain flavonoids and shows the activity as a type 2 anti-diabetic by a mechanism of inhibiting the absorption of glucose. Flavonoid compounds such as rutin, quercetin and hesperidin shows anti-diabetic activity. This research aims to determine the levels of total flavonoids and docking studies of flavonoids from P. niruri against ?-amylase and ?-glucosidase. Analysis of total flavonoid content utilizes colorimetric method using AlCl3 and rutin standard, the docking study uses Autodock Vina program with the assistance of AutoDockTools (ADT) and visualization of docking results using Discovery Study. The results of the determination of total flavonoids level was 12.32±0.53 mgRE/g extract whereas in ethyl acetate and water fraction each were 19.33±0.68 and 20.07±1.23 mgRE/g, respectively. Results of all flavonoids docking study showed the potential to bind to both receptors, rutin has better binding energy towards ?-amylase whereas against ?-glucosidase is demonstrated by 3-O -?-D-glucopyranosyl-(2-1)-O-?-D-xylopyranoside quercetin.
