| Abstrak/Abstract |
Mistletoe has been acknowledged as medicinal plant for traditional treatment because it possesses beneficial compound which
potentially aid various diseases. Despite its destructive nature, each species of mistletoes can exhibit different beneficial
compounds. Exploratory research has been conducted for detection secondary metabolites of Dendrophthoe pentandra that grow
on the Bodhi tree (Ficus religiosa L.) at Universitas Gadjah Mada, Yogyakarta, Indonesia. The aim of this research is to detect
secondary metabolites which potentially for medicational purpose in D. pentandra using thin layer chromatography method. The
mistletoes sample was obtained from the Bodhi tree, authentication of the species of mistletoes was carried out at the Department
of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada. D. pentandra leaf was washed and dried in a dryer
cabinet at 50o C, it was subsequently powdered. Amount of 5 g of leaf powder was macerated with 50 ml of 95% ethanol. Analysis
of chemical compounds was carried out using the Thin Layer Chromatography (TLC) method with silica gel 60 F254 as a
stationary phase and a mobile phase suitable for terpenoids and flavonoids compounds. The mobile phase of n-hexane: ethyl
acetate (93:7) was used for terpenoid detection, meanwhile ethyl acetate: formic acid: water (90:5:5) were used for flavonoid
detection. The result was further investigated under visible light and UV light 254 & 366 nm followed by anisaldehyde-H2SO4
spray reagent and stigmasterol as standard for terpenoid detection, and sitroborat spray reagent with quercitrin standard for
flavonoid detection. Based on detection by TLC method, ethanolic extract D. pentandra leaves did not contain any alkaloids. D.
pentandra contains stigmasterol which is indicated by pink spots after being sprayed with sulfuric acid anisaldehyde (hRf 70). The
results of the study also showed that D. pentandra contained quercitrin which was indicated by yellowish green spots after being
sprayed with sitroborate (hRf 60) and observed under 366 nm UV lamp. |
