Water-Soluble Fiber from Bengkoang (Pachyrhizus erosus (L.) Urban) Tuber Modulates Immune System Activity in Male Mice
Penulis/Author
HANIF NASIATUL BAROROH (1); Prof. Dr. apt. Agung Endro Nugroho, S.Si., M.Si. (2); Prof. Dr.rer.nat. apt. Raden Rara Endang Lukitaningsih, S.Si., M.Si. (3); Prof. Dr. apt. Arief Nurrochmad, M.Si., M.Sc. (4)
Tanggal/Date
10 2020
Kata Kunci/Keyword
Abstrak/Abstract
Bengkoang (Pachyrizus erosus (L.) Urban) tuber contains fiber and has the potential to be developed as an immunomodulator. The present study was performed to evaluate the immune-modulating effects of a water-soluble fiber of P. erosus in mice induced hepatitis B vaccine. Thirty mice were divided into six groups that induced by hepatitis B vaccine intraperitoneally (i.p) at day 7 and 14. The mice were treated by bengkoang fiber extract (BFE) and bengkoang fiber fraction B (BFE-B), levamisole, and sodium carboxymethyl cellulose for 18 days. At the end of treatments (day 19), phagocytic activity, lymphocyte proliferation, spleen index, cytokine, and IgG production were determined. Our results showed that water-soluble fiber significantly increased phagocytic capacity, nitrite oxide secretion, and spleen index. BFE-B could modulate TNF ? and IL-10 secretion, but BFE had no effect on cytokine production. The lymphocyte proliferation assay showed that BFE 50 mg/kg BW and BFE-B 50 mg/kg BW significantly enhanced lymphocyte proliferation. The BFE-B (25 and 50) mg/kg BW stimulated Immunoglobulin G production. In conclusion, our results indicate that the bengkoang (P.erosus) fiber fraction (BFE-B) had an immune-modulating effect in innate and adaptive immune responses while fiber extract BFE modulated innate immune response.
