The use of Fe3O4 magnetic nanoparticles as the active layer to detect plant's DNA with surface plasmon resonance (SPR) based biosensor
Penulis/Author
Devy Pramudyah Wardani, S.Si., M.Sc. (2); Prof. Dr.Eng. Edi Suharyadi, S.Si., M.Eng. (3); Prof. Dr. Kamsul Abraha (4)
Tanggal/Date
2016
Kata Kunci/Keyword
Abstrak/Abstract
The surface plasmon resonance (SPR) phenomenon has been observed on the silver-coated prism and Fe3O4
magnetic nanoparticles for detecting plant’s DNA. Fe3O4 nanoparticles were synthesized using temperature of 30°C and
they were coated with PEG4000. The composite of Fe3O4+PEG4000 has a function as a DNA binding active layer and
for optimizing the detection ability of SPR. SPR-based biosensor in this work using Kretschmann configuration and He-
Ne laser beam (? = 632.8 nm). The plant’s DNA used in this study has ratio 1.828. The plant’s DNA could be detected by
SPR-based biosensor with the ratio of composite Fe3O4 : PEG4000 is 1:1. This work was done for the earlier study to
approve that SPR can be used to detect DNA with the active layer on it (in this case: composite Fe3O4+PEG4000). Thus,
the shift of SPR-angle was influenced by the DNA ratio, concentration of Fe3O4 and its refractive index.
