| Abstrak/Abstract |
This study was conducted in Musi river area to assess the extent of ammonia-oxidizing
bacteria (AOB) diversity as affected by land-use activities. PCR-Terminal Restriction Fragment Length
Polymorphism (PCR-TRFLP) analysis of 16S rRNA gene was used to evaluate the diversity of AOB. A total
of 158 Terminal Restriction Fragments (TRFs) of AOB in different sizes digested with BsuRI and MspI
were obtained from all samples that were aligned to representative published sequences of 13 strains.
The number of TRFs in each sample ranged from 52 to 491. There were eight strains of AOB results of
digesting the two-restriction enzymes within the beta-subdivision ammonia-oxidizers namely
Nitrosomonas europaea, Nitrosomonas eutropha, Nitrosomonas ureae, Nitrosomonas europaea strain C-
31, Nitrosospira briensis, Nitrosospira briensis Nsp10, Nitrosococcus mobilis Nc2, and Nitrosolobus
multiformis, its had the similarity coefficient in the range of 93-96%. The environmental conditions that
affect the diversity of AOB are salinity, temperature, DO, and nutrients. |
