The Short Communication: Polymorphism of Myostatin Gene and its Association with Body Weight Traits in A Hybrid of GAMA Chicken (Gallus Gallus domesticus Linn. 1758)
Penulis/Author
TANJUNG ARDO (1); Dr.med.vet. drh. Hendry T.S.S.G. Saragih, M.P. (2); Drs. Trijoko, M.Si. (3); Soenarwan Hery Poerwanto, S.Si., M.Kes. (4); Dr. Slamet Widiyanto, S.Si., M.Sc. (5); I WAYAN S M (6); Prof. Dr. Budi S. Daryono, M.Agr.Sc. (7)
Tanggal/Date
11 2019
Kata Kunci/Keyword
Abstrak/Abstract
Abstract. TanjungA, Saragih HTSSG, Trijoko, Soenarwan HP, Widianto S, Mahardhika IWS, Daryono BS. 2019. Polymorphism of myostatingene and its association with body weight traits in a hybrid of GAMA chicken(Gallus gallus domesticusLinn. 1758).Biodiversitas 20: 3207-3212.An experiment was conducted to detect SNP of themyostatingene and its association with the body weightof hybrid chicken crossbreed from F1Kamperand BC1Broiler.Four F1Kamperhens were crossbred with BC1Broiler rooster. Dayold chick(DOC)hatched were maintained for 49 days with body weightmeasurement every seven days. The blood samples from 49 days oldchicken were taken for DNA isolation by Chelex 5% method and then amplification of the myostatingene. PCR productswere sequenced, and sequence alignment was performed using Clustal Omega to obtain SNP. The SNP obtained was analyzed by the Pearson correlation test with the body weightof forty nine-days-old chickens.The body weightof the hybrid chicken is higher than of Pelungchicken but lower than the Broiler. There are 7 SNPs in myostatingene exons included 2 Adenine insertions, 1 Guanine deletion, and four substitutions(C2244G, G2283A, T4842G, G7378T) that yield nine haplotypes. Six haplotypes had different protein sequences withMyostatinprotein, while three haplotypes were identical toMyostatinprotein. The correlation analysis showed that there was a strong positive correlation(r = 0.736) between normalMyostatinprotein and mutant to chicken body weight at 49-days-old. Adenine insertion to nucleotide 2099-2100 of myostatingene had a very strong positive correlation(r = 0.800) to 49-days-old chicken body weight, although T4842G substitution had a strong negative relationship(r = -0.773) to 49-days-old chicken body weight.Adenine insertion to nucleotide 2099-2100 of myostatingene could be a genetic marker of heavier body weightof the hybrid chicken
Rumpun Ilmu
Biologi (dan Bioteknologi Umum)
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
4146-Article Text-12768-1-10-20191018.pdf
[PAK] Full Dokumen
2
Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn_ 1758).pdf
