| Abstrak/Abstract |
Biofilms play an essential role in chronic infections and can resist antibiotics, indicating the need
to develop novel antibacterial and antibiofilm agents using new plant sources. Kulim
(Scorodocarpus borneensis Becc.), which is endemic to Borneo and widely known for its
distinctive garlic-like aroma, and empirical medicinal use. This study aims to investigate the
antibacterial and antibiofilm properties of Kulim leaves hydrosol. Hydrosol was obtained through
distillation, and its phytochemical composition was characterized using GC-MS. Antibacterial and
antibiofilm activities were then assessed using microdilution assays to determine MIC₅₀, MBIC₅₀,
and MBEC₅₀. This study also examined the impact of Kulim hydrosol on the motility of
Pseudomonas aeruginosa, a critical virulence factor in biofilm formation and infection
persistence. The main compound identified was di-2-propenyl (CAS) allyl trisulfide. The MIC50
values for S. aureus, P. aeruginosa, E. coli, and polymicrobial growth were 79.19%, 61.74%,
93.07%, and 67.58% (v/v), respectively. The MBIC50 values for these bacteria were 71.68%,
36.45%, 73.71%, and 61.70% (v/v), respectively. In addition, the MBEC50 values at 24 hours were
66.24%, 61.37%, 75.76%, and 39.15% (v/v), respectively. The MBEC50 at 48 hours were
determined to be 60.73%, 64%, 70.70%, and 56.47% (v/v), respectively. Kulim hydrosol was also
reported to inhibit 50% of P. aeruginosa swimming, swarming, and twitching motility at rates of
22.96%, 19.36%, and 41.99% (v/v), respectively. These results suggest that the test sample has
potential as an antibacterial, antibiofilm, and antimotility agent, making it valuable for application
in food preservation, healthcare, and industry. |
