Karya
Judul/Title The neuroprotective effect of Ocimum sanctum Linn. ethanolic extract on human embryonic kidney-293 cells as in vitro model of neurodegenerative disease
Penulis/Author Puspa Hening (1); MADE BAGUS AURIVA M (2); Dr.biol.hom. Nastiti Wijayanti, S.Si., M.Si. (3); Prof. drh. Dwi Liliek Kusindarta, MP., Ph.D. (4); Dr. med. vet. drh. Hevi Wihadmadyatami, M.Sc. (5)
Tanggal/Date 11 2018
Kata Kunci/Keyword
Abstrak/Abstract AIM: This study aimed to analyze the neuroprotective effect of Ocimum sanctum Linn. ethanolic extract (OSE) on human embryonic kidney-293 (HEK-293) cells as the in vitro model of neurodegenerative diseases. MATERIALS AND METHODS: In this research, HEK-293 cells divided into five groups consisting of normal and healthy cells (NT), cells treated with Camptothecin 500 µM as the negative control, cells treated with trimethyltin 10 µM (TMT), cells treated with OSE 75 µg/ml, and cells pre-treated with OSE 75 µg/ml then induced by TMT 10 µM (OSE+TMT). MTT assay and phase contrast microscopy were applied to observe the cell viability quantitatively and morphological after Ocimum sanctum Linn extract treatment. Finally, the reverse transcription polymerase chain reaction was employed to study the expression of choline acetyltransferase (ChAT). RESULTS: The MTT assay and phase contrast microscopy showed that OSE pre-treatment significantly increased the viability of TMT-induced apoptotic cells and maintained cell viability of the normal HEK-293 cells. Expression of ChAT markedly reduced on TMT treatment group, but OSE administration stabilized ChAT expression in TMT-induced HEK-293 cells. CONCLUSION: This present study proved that OSE administration has neuroprotective effect by increased HEK-293 cells viability and maintain ChAT expression.
Rumpun Ilmu Kedokteran Hewan
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1The neuroprotective effect of Ocimum sanctum Linn ethanolic extract.pdf[PAK] Cek Similarity
2Artikel Jurnal_The neuroprotective effect of Ocimum sanctum Linn.pdf[PAK] Full Dokumen
3Korespondensi Vet World HEK 293-Neurodeg.pdf[PAK] Bukti Korespondensi Penulis
4Front Matters Veterinary World Sept 2018 .pdf[PAK] Halaman Cover
5PEER REVIEW_Hevi 17.pdf[PAK] Peer Review
62018_Hening_SuKet_Publikasi_Nastiti Wijayanti.pdfDokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)