| Penulis/Author |
HASYRUL HAMZAH (1) ; Prof. Dr.rer.nat. apt. Triana Hertiani, S.Si., M.Si. (2); Dr. Sylvia Utami Tunjung Pratiwi, S.Si., M.Si. (3); Prof. dr. Titik Nuryastuti, M.Si., Ph.D., Sp.MK(K). (4) |
| Abstrak/Abstract |
Biofilm acts as the mediator for infection nowadays. Approximately, more than 80% infection
incidents are biofilm-formation related. Biofilm as bacteria's defense system is more difficult to eradicate
by antibiotic; therefore, pathogen bacteria on their biofilm forms can make serious problems for human
health. The invention of a new candidate for polymicrobial biofilm can be an essential challenge to be
studied, in order to prevent infections related to biofilm. Tannin is a polyphenol compound with antibacterial and anti-fungal potential. This study aims to acknowledge the effectiveness of tannin in inhibition
and degradation of C. albicans, P. aeruginosa, E. coli, S. aureus, and polymicrobial biofilm. The assay for
biofilm inhibition and degradation were determined with microtiter broth method. The effectivity of tannin
antibiofilm against polymicrobial biofilm were analyzed by calculating minimum biofilm inhibitory
concentration (MBIC50) and minimum biofilm eradication concentration (MBEC50) values. The mechanism
of action of tannin against polymicrobial biofilm was tested using scanning electron microscopy (SEM). The
data were analyzed using the Statistical Package for the Social Sciences (SPSS) with a 95% confidence level.
Tannin 1% gave inhibition activity of mono-species biofilm formation S. aureus in the middle phase and
maturation of 79.04±0.01, 61.48±0.03, E. coli 74.56±0.01, 67.91±0.02, P. aeruginosa 67.32±0.05, 35.13±
0.01, C. albicans 60.62±0.01, 47.16±0.01. The results also provide evidence that tannin activity can degrade
and damage the matrix of extracellular polymeric substance (EPS) polymicrobial biofilms. Hence, tannins
can be a potential candidate for new antibiofilm for polymicrobial biofilm.
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