The Influence of Protein Level on Feed with the Ovarian Follicle Development of Laying Hens Gallus gallus
Penulis/Author
Indra Lesmana, S.Si., M.Sc. (2); Dr.med.vet. drh. Hendry T.S.S.G. Saragih, M.P. (3); Prof. Dr. Budi S. Daryono, M.Agr.Sc. (4); Drs. Trijoko, M.Si. (5)
Tanggal/Date
2017
Kata Kunci/Keyword
Abstrak/Abstract
Laying chicken attracts interest from farmers because of its high egg
production. Feed quality and quantity determine chicken egg production level. The
purpose of this study is to determine the influence of protein level on feed into the
development of follicles in laying hens. This research used 20 individuals of dayold
chick (DOC) Lohmann Brown, divided into four groups with five individuals in
each group. In the first treatment, chickens are reared for 7 weeks and being fed with
21% protein level. In the second treatment, chickens are reared for 7 weeks and
being fed with a protein level of 14%. In the third treatment, chickens are reared for
8 weeks and being fed with 21% protein level and for 8 weeks with 14% protein
level at the fourth treatment. Body weight was measured every week. In the seventh
and eighth week, each group was dissected for the organ harvesting and tissues
processing. Ovaries were prepared and weighted to observe development of the follicle
using hematoxylin-eosin staining and immunohistochemistry (IHC). Follicular
morphology can be classified and quantified serially on each slide. Data were analyzed
by T-test to see its significance (P < 0.05). The results of the study explained
that the treatment with 21% protein level on feeding increases primordial and secondary
follicle development in the seventh and eighth week significantly compared
with 14% protein level. At the age of 8 weeks, there are an increasing number of
follicle developments significantly on 21% protein level group compared with 14%
protein level group.
