The impact of COL1A1 and COL6A1 expression on hypospadias and penile curvature severity
Penulis/Author
Dr. dr. Prahara Yuri, Sp.U(K). (1); Prof. dr. Gunadi, Ph.D, Sp.BA., Subsp.DA(K) (2); RAHMADANI PUJI LESTARI (3); FIRLY PUTRI FARDILLA (4); Wiwit Ananda Wahyu Setyaningsih, S.Keb., M.Sc. (5); dr. Nur Arfian, Ph.D. (6); Dr. dr. Ishandono Dachlan, M.Sc., Sp.B., Sp.BP-RE(K) (7)
Tanggal/Date
1 2020
Kata Kunci/Keyword
Abstrak/Abstract
Abstract
Background: Hypospadias, the most frequent congenital male external genitalia abnormality, is usually associated with curvature of the ventral penis, i.e. chordee. Abnormality of dartos tissue has been suggested as the pathophysiology of chordee. Collagen is one of the most abundant fibrous proteins within the extracellular matrix. In this study, we determined the expressions of collagen 1 (COL1A1) and COL6A1 in patients with hypospadias and associated them with the severity of penile curvature.
Methods: We involved 60 children <18>60 degree).
Results: qPCR showed that the COL1A1 and COL6A1 expressions were significantly downregulated in distal (0.88 (0.38-2.53) and 0.54 (0.16-4.35), respectively) and proximal 0.76 (0.33-2.57) and 0.57 (0.18-1.38), respectively) hypospadias groups compared to controls (1.85 (0.24-4.61) and 0.93 (0.17-4.06), respectively) with p -values of 0.024 and 0.018, respectively. Furthermore, there was a moderate correlation between COL1A1 and COL6A1 expressions ( r= 0.458, p< 0.0001). Interestingly, the COL1A1 and COL6A1 were also significantly downregulated in the moderate and severe compared to the mild chordee groups with p -values of 0.003 and 0.037, respectively.
Conclusions: Aberrant COL1A1 and COL6A1 expressions might affect the abnormality of dartos tissue and penile curvature severity in hypospadias patients.
Rumpun Ilmu
Bedah (Umum, Plastik, Orthopaedi, Urologi, Dll)
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
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The impact of COL1A1 and COL6A1 expression on hypospadias and penile curvature severity_2.pdf
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