| Abstrak/Abstract |
Plant's response to drought occurs through a series of mechanisms that involve transcription regulation. This research was conducted to study transcription factors (TF) in the drought response of local rice of East Nusa Tenggara (Nusa Tenggara Timur, NTT) and physiological changes during drought stress. By using three NTT local rice cultivars: Boawae Seratus Malam (BSM), Gogo Jak (GJ), and Kisol Manggarai (KM) and the fraction of transpirable soil water (FTSW) method with two treatment levels: FTSW 1 (control) and FTSW 0.2 (severe stress) we analyzed the TF expression of OsDREB1A, OsDREB2A, OsWRKY45, and OsNAC6. Based on data, the highest level of TFs expression occurred in BSM cultivars, followed by GJ and KM. The analysis of physiological characters showed an association between TFs expression levels and physiological responses; BSM cultivar showed high pigment levels, high proline contents, and lower H2O2 levels. A linkage was also found in water conservation as indicated by the higher relative water content and cell membrane stability index in BSM cultivar, in contrast to lower electronic leakage and malondialdehyde percentage when exposed to drought. Based on the results, it can be concluded that BSM cultivar can be considered as drought tolerant local cultivar according to morpho-physiological analysis. In this study, as response to drought stress, all NTT local rice cultivars showed subtle upregulation of stress responsive transcription factors OsDREB1A, OsDREB2A, OsWRKY45, and OsNAC6. |
