The effects of Hydnophytum formicarum ethanolic extract towards lymphocyte, vero and T47d cells proliferation in vitro
Penulis/Author
Prof. Dr.rer.nat. apt. Triana Hertiani, S.Si., M.Si. (1); Prof. Dr. Dra. apt. Ediati, SE. (2)
Tanggal/Date
2014
Kata Kunci/Keyword
Abstrak/Abstract
This research aims were to explore the effects of Hydnophytum formicarum Jack (Rubiaceae) known as the Ant
plant on proliferations of mouse lymphocyte, Vero and T47D's cell lines. Plant hypocotyls were collected from
West Papua. Some parts were dried to gain plant with ant residue (A), while other parts were separated from the
ant residue to gain plant without ant residue (B) and ant residue (C). After being macerated in ethanol and
evaporated to dryness, each extract was tested on Balb/c mice lymphocytes and Vero cell lines by MTT assay in
the presence and absence of Doxorubicin (Dox). Effect on T47D was observed by using Dox 25 ?g/mL as
control. Data obtained were analyzed by ANOVA and paired sample T-test. Thin layer chromatography profiles
were evaluated to identify the difference in chemical contents. The extracts A and B had the ability to increase
lymphocyte proliferation by increasing concentration. Both extracts could not significantly enhanced the
proliferation of Vero cells, neither in the presence nor absence of Dox. The extract B in 100 ?g/mL showed the
highest inhibition against T47D cells by 53.45%. There was no chemical content difference observed on extracts
A and B
