| Abstrak/Abstract |
"Objective: To investigate the effect of furosemide on kidney damage in AKI rat models.
Methods: Twenty-five male (2-3 months old) Sprague-Dawley rats were used in this study. They divided into 5 groups; sham operation (SO, n=5), ischemic-reperfusion (IR, n=5), IR+furosemide 3.6 mg/kgBW (IR+F1, n=5), IR+furosemide 7.2 mg/kgBW (IR+F2, n=5), and IR+furosemide 14.4 mg/kgBW (IR+F3, n=5). Abdominal surgery was performed under ketamine anesthesia to produce ischemic reperfusion (IR) by mean of renal artery clamping for 45 minutes. Urine output, serum creatinine level, tubular injury score, and TLR4 gene expression were examined to investigate kidney damage. Periodic Acid-Schiff (PAS) staining was measured to examine kidney tubular injury. Data were analyzed using One-Way ANOVA (p<0.05).
Results: AKI rat models which were given 3.6 mg/kgBW and 7.2 mg/kgBW of furosemide (0.014±0.001; and 0.012±0.007) showed higher (p>0.05) creatinine clearance compared to IR (0.009±0.003) while administration of 14.4 mg/kgBW furosemide (0.009±0.004) denoted equal creatinine clearance to IR (p>0,05). Kidney tubular injury score of 3.6 mg/kgBW furosemide (2.89±0.13) was lower (p>0.05) than IR (3.26±0.19) whereas 7.2 mg/kgBW and 14,4 mg/kgBW furosemide (3.55±0.26; 3.83±0.19) were higher (p<0.05) than IR. Administration of 3.6 mg/kgBW furosemide (0.99±0.08) indicated lower (p<0.05) TLR4 gene expression than IR (1.20±0.08) whilst 7.2 mg/kgBW furosemide (1.23±0.13) was not-significantly higher (p>0.05) and 14.4 mg/kgBW furosemide (1.63±0.12) was significantly higher (p<0.05) than IR.
Conclusion: Administration of 3.6 mg/kgBW furosemide reduced kidney damage in AKI rat models while higher dosages (7.2 mg/kgBW and 14.4 mg/kgBW) increased kidney damage in the used models."
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