Karya
Judul/Title The effects of dissolved oxygen tension and ammonium concentration on polyhydroxybutirate synthesis from cassava starch by Bacillus cereus IFO 13690
Penulis/Author Dr. Margono, S.T., M.T. (1) ; Prof. Ir. Rochmadi, S.U., Ph.D., IPU. (2); Ir. Siti Syamsiah, Ph.D. (3); Dr. Ir. Muhammad Nur Cahyanto, M.Sc. (4)
Tanggal/Date 2009
Kata Kunci/Keyword
Abstrak/Abstract Attempting to get low price of raw material for producing polyhydroxybutyrate is always studied. Tapioca starch is one of the raw material with low price. The objective of this research was to study the effects of initial ammonium concentration and dissolved oxygen tension (doT) on producing PHB by Bacillus cereus IFO 13690 with tapioca starch as the carbon source. This fermentation was carried out in 5 L fementors with a 2 L working volume, temperature of 30 oC, and agitation of 500 rpm. The pH medium was controlled at 5.6 after it came down from the initial pH of 6.8. Meanwhile, the initial doT was 100 % air saturation and also came down to and maintained at doT of experiment, i.e. 1 , 5 , or 10 % air saturation. The best result was obtained when the initial ammonium concentration was 5 g/L and the doT value maintained at 5 % air saturation. By this conditions, the cell growth reached 5,457 g cell dry weight/L containing PHB of 2.42 ?ll dry weigh after 29 hours fermentation.
Rumpun Ilmu Teknologi Hasil Pertanian
Bahasa Asli/Original Language English
Level Nasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
12009_06_IJB_14(1)_1139_PHB.pdf[PAK] Full Dokumen
2SK Akreditasi IJBiotech 2020-2024.pdfDokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)