| Penulis/Author |
Widya Asmarawati, S.Pt., M.Sc. (1); Dr. Ir. Kustono, M.Sc. (2); Prof. Ir. Diah Tri Widayati, MP., Ph.D., IPM (3); Dr. Ir. Sigit Bintara, M.Si., IPU., ASEAN Eng. (4); Prof. Ir. Ismaya, M.Sc., Ph.D. (5) |
| Abstrak/Abstract |
This research conducted to determine the influence of various cryoprotectants and diluents on post-thawing semen quality. Two diluent used were buffer phosphate (BP) and egg-yolk buffer phosphate (EYBP). Three cryoprotectants added to the diluents were glycerol (G), dimethyl formamide (DMF) and dimethyl sulfoxide (DMSO). Semen were collected from 4 males chicken aged 1 year to 1.5 years. After examined, 2 different diluents and 3 different cryoprotectants were adding into polled semen that was devided into 6 different treatment tubes (BP+G 7%, BP+DMF7%, BP+DMSO7%, EYBP+G 7%, EYBP+DMF7%, and EYBP+DMSO7%), then prosessed to be frozen with slow freezing method. Motility before dan after equilibration were examined. Frozen semen was stored at temperature -196 °C for a week, then thawed and its motility were examined. Fresh sperm quality were analyzed with mean and standard deviation, whereas data of motility before equilibration, after equilibration, and post-thawing were analyzed by analysis of variance using completely randomized design factorial 2 x 3. The result showed that diluents treatment gave significant differences (P?0.05) on sperm motility after equilibration and post-thawing, but not gave significant differences on sperm motility before equilibration. Cryoprotectant treatments gave significant differences (P?0.01) on sperm motility before equilibration and after equilibration, but not gave significant differences on post-thawing sperm motility. It can be concluded that DMF adding into EYBP diluents gave highest post-thawing sperm motility. |
