| Abstrak/Abstract |
Dendrobium phalaenopsis Fitzg. (also known as the Larat orchid) is an endemic orchid from Larat Island, Eastern
Indonesia. Its beautiful flowers mean that many plants are taken for commercial purposes, leading to the rapid decline
of populations in their natural habitats. The objectives of this study were to determine which organs of the transgenic
Larat orchid carrying the 35S::GR::AtRKD4 construct, together with which concentrations of the plant growth regulators
(PGRs) auxin and cytokinin, are suitable for the induction of somatic embryos (SEs). In this study, the AtRKD4 gene in Larat
orchids was confirmed using PCR with specific primers for the AtRKD4 and HPT genes. Thidiazuron (TDZ) (1, 3 and 5 mg/L)
in combination with 1‐naphthaleneacetic acid (NAA) (0.5 and 1 mg/L) were used on new phalaenopsis (NP) medium to
induce SEs from leaves, pseudobulbs and roots. The AtRKD4 transgenes were detected as being stably integrated into the
DNA genome of transformant plants using specific primers for AtRKD4 and HPT genes, and positive results were obtained
using ACTIN gene primers as internal controls for PCR. Pseudobulbs produced 19 to 20 SEs from 108 pseudobulb explants
(89–100%), a higher number than produced in explants of the other organs studied. Among the PGR treatments, the best
results were obtained in NP medium supplemented with a combination of 1 mg/L TDZ and 1 mg/L NAA, 100% of the
explants of which produced SEs (2.11 ± 1.36). No significant difference was found between the morphology of the SEs
produced from the non‐transformant Larat orchid pseudobulb explants and the 35S::AtRKD4 carrier transformant. |
