The Effectiveness and Bacterial Communities of Biofertilizer Application for Huanglongbing Disease Control in Indonesia
Penulis/Author
Sri Widyaningsih (1); Prof. Dr. Tri Joko, S.P., M.Sc. (2); Prof. Dr. Ir. Sri Nuryani Hidayah Utami, M.P., M.Sc. (3); Prof. Dr. Ir. Siti Subandiyah, M.Agr.Sc. (4)
Tanggal/Date
2018
Kata Kunci/Keyword
Abstrak/Abstract
Huanglongbing (HLB) which is caused by Candidatus liberibacter asiaticus (CLas) is one of
the citrus diseases that cause the largest losses on citrus orchards in Indonesia and other countries.
An experiment was conducted at the HLB endemic area in Purworejo district of Central Java to
study the response of biofertilizer treatment on HLB susceptible citrus plant (Citrus reticulata)
against the disease. The trial was using graft inoculated and non-inoculated by inoculum HLB.
Plants treated by biofertilizer A (manufactured) or biofertilizer B (farmer’s formulation), once
a month during 3 years and 8 months. CLas detection was conducted in the last 9 months. The
results showed that application of biofertilizer B was effective in reducing HLB disease naturally
transmitted by vector (AUDPC value of 195.24 by leaf score and 218.58 by canopy score).
Biofertilizer A was effective to control HLB disease on HLB-inoculated plants. Detection of
CLas by PCR (Polymerase Chain Reaction) show that some plants have instability in the detection
results that were influenced by the concentration of CLas. The rhizobacteria population in plant
without the HLB inoculation and applied biofertilizer A were higher than in plants with the HLB
inoculation and applied biofertilizer B. The total population of rhizobacteria tended to increase
except in the treatment of biofertilizer A, that were applied on HLB-inoculated plants. The
application of biofertilizer to artificially infected plants and naturally transmitted through vectors
were able to prolong the viability of the plant.
