| Penulis/Author |
Dr. Ir. Sigit Bintara, M.Si., IPU., ASEAN Eng. (1) ; Prof. Ir. Ismaya, M.Sc., Ph.D. (2); Prof. Ir. Diah Tri Widayati, MP., Ph.D., IPM (3); Ir. Riyan Nugroho Aji, S.Pt., M.Sc., IPP (4); Widya Asmarawati, S.Pt., M.Sc. (5) |
| Abstrak/Abstract |
Artificial insemination supports the prevention of infectious dispersion in organisms which share the same environment. Meanwhile, spermatozoa usually experience a decrease in quality during processing due to the peroxidation process. This study aims to identify the effect of vitamin E antioxidant addition in goat milk diluent on the quality of thin-tailed sheep semen after storing at 5C for five days. A total of 4 male thin-tailed sheep were used and the treatments consisted of controls, namely goat milk sperm diluent without vitamin E antioxidant (P0), and others with the addition of vitamin E at a dose of 2 gL-1 diluent (P1); 3 gL-1diluent (P2); and 4 gL-1 (P3). Moreover, the variables observed include individual motility, viability, and intact plasma membrane of spermatozoa after storing at 5C for five days. The data were analyzed statistically using analysis of variance (ANOVA) with a completely randomized design in one-way. The results showed that the addition of vitamin E antioxidant to goat milk diluent during sperm dilution had a significant effect (P < 0.05) on the motility, viability, and intact plasma membrane. Spermatozoa motility at P0, P1, P2, and P3 were 44.0%, 48.2%, 50.4%, and 53.2%, while the viability was 48.2%, 51.4%, 54.2%, and 56.0%, respectively. Additionally, the intact plasma membranes at P0, P1, P2, and P3 were 41.0%, 46.6%, 48.2%, and 51.4%, respectively. The addition of antioxidant vitamins in goat milk diluent with a concentration of 4 gL-1 produced the best results on sperm quality after cooling. Based on the results, it was concluded that the addition of vitamin E antioxidant to the sperm diluent can improve the quality of thin-tailed sheep chilled semen. |
