Karya
Judul/Title The effect of photoperiod on lipid production of mixed culture Arthrospira maxima Setchell et Gardner and microalgae consortium of glagah isolate
Penulis/Author DITA KANTI MAHARANI (1); AHMAD ARDI (2); ANDI ALFI SYAHRIN (3); Thoriq Teja Samudra (4); Ir. Yano Surya Pradana, S.T., M.Eng., IPM., ASEAN Eng., ACPE., APEC Eng. (5); Prof. Dr.Eng. Ir. Arief Budiman, M.S., IPU. (6); Dr. Eko Agus Suyono, S.Si., M.App.Sc. (7)
Tanggal/Date 2020
Kata Kunci/Keyword
Abstrak/Abstract Microalgae are organisms with huge potential to be a source of biodiesel production. Various types of microalgae have different capacities to produce lipids. Mixed cultures such as Arthrospira maxima and microalgae consortium of Glagah isolate are known to produce lipids higher than single culture. Light is an important factor for microalgae, it was needed as a source of energy in photosynthesis. And photosynthesis was the key to hold metabolic processes in microalgae. Light factors that can affect the photosynthesis include light intensity, wavelength and duration of radiation (photoperiod). The longer illuminat ion will boost the growth of microalgae cell. In the microalgae industry, especially in indoor light is a factor that takes up a lot of product ion costs. So the use of light energy should be made as efficient as possible to reduce the cost of production. Therefore, this study aimed to know the duration of radiation that most efficiently promoted cell growth and lipid production of mixed cultures A. maxima and microalgae consortium of Glagah isolate. Variations in the light-dark photoperiod used were 12-12, 16-8, 20-4, and 24-0 hours. The method used to calculate the density of cells was using Haemocytometer and for lipid analysis was using the method of Bligh and Dyer. The results obtained showed that the 16-8 hour light -dark treatments could generate cell density and lipid production with the highest which each value were 5.32 million cell·mL?1 and 0.27 mg·mL?1, respectively with the percentage of lipid by dryweight was 29.57%. In conclusion, the photoperiod 16 hours of light - 8 hours of dark produce the lipid content of mixed culture of A. maxima and a consortium of microalgae Glagah isolates which reaches 0.27 mg·mL?1. In other words, the product ion of lipids produced from this mixed culture is 29.57% of its dry weight. The 16 hours of light photoperiod - 8 hours dark produces the highest number of mixed cultures of A. maxima and a microalgae consortium of Glagah isolates. The relationship between photoperiod with other factors, such as light intensity, temperature, and nutrients were needed to obtain an optimal lipid production of A. maxima and the consortium of Glagah isolates.
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