| Abstrak/Abstract |
Cellulite is a normal condition judging from the medical aspect, but from
aest
hetics aspect, cellulite deserves more attention, especially for women.
Centella
asiatica
is reported to promote both fibronectin and collagen synthesis. Meanwhile,
Zingiber officinale
is reported to have lipolysis activity. Combination of the two herbals
is assumed to have complementary effect as anti cellulite agents. The combination of the
herbal extracts is prepared in nanoemulsion form to enhance the bioavailability. The
study aimed to determine the effect of nano emulsion of
C. asiatica
and
Z. officin
ale
combination (proportion of 5:1) to stimulate skin collagen synthesis and decrease the
diameter of adipocyte cells using histological parameters as an indicator of lipolysis
activity. Twenty female Wistar rats weighing 120
-
140 g were fed a high
-
fat diet
for 30
days. The animals were divided into 4 groups, with the 3 groups received the
nanoemulsion of herbal combination with the dose of 50, 100, and 200 mg/kg BW for 30
days, and 1 group received CMC Na 0, 5% as negative control. On the day 31, rats were
sacrified and skin samples of 1.0 cm
2
, including fatty tissue, were obtained from the
subjects. One part of the tissue sample was used for collagen assay, while another was
used for hematoxylin
-
eosin staining. The amount of collagen in skin tissue was ass
ayed
using Sirius Red Collagen Detection Kit (Chondrex). The collagen thickness was also
measured histologically using Sirius red staining. The diameter of adipocyte cells were
measured under light microscope to represent lipolysis activity. Results showed
that the
amount of skin collagen was increased with the increase of extract doses, however the
lowest dose showed no significant different with the normal animal. The diameter of
adipocyte cells was also decreased in dose
-
dependent manner. Results indicat
e that the
combination of
C. asiatica
and
Z. officinale
can be developed as herbal medicine for anti
cellulite agent
. |
