Protective Effect of Microbubble Aeration and Dietary Probiotics BALSS on Survival and Immunity of White Leg Shrimp (Litopenaeus vannamei) Postlarvae against Acute Low Salinity Stress
Penulis/Author
SHIMA BHASKARA A (1); Indah Istiqomah, S.Pi., M.Si., Ph.D (2); Prof. Dr. Ir. Rustadi, M.Sc. (3); Dr. Ir. Bambang Triyatmo, M.P. (4); Prof. Dr. Ir. Alim Isnansetyo, M.Sc. (5); Prof. Wiratni, S.T., M.T., Ph.D. (6); Prof. Dr. Eng. Ir. Deendarlianto, S.T., M.Eng. (7)
Tanggal/Date
3 2019
Kata Kunci/Keyword
Abstrak/Abstract
Survival rate and immune parameters of whiteleg shrimp ((Litopenaeus vannamei) postlarvae against salinity stress were evaluated in an indoor experimental trials. The research was conducted with 2 x 2 factorial design with two levels of aeration (micro bubble and macro bubble) and two levels of diet (with and without probiotics) resulting four treatments in triplicate: application of micro bubble with gut probiotics (MiP), without gut probiotics (MiC), and macro bubble with gut probiotics (MaP) and without probiotics (MaC). Vaname shrimps measuring 7.6 g were maintained in the tanks for 60 days and fed five times a day at a dose of 5% biomass. Harvested vaname shrimp were tested for low salinity stress (5 ppt) for 3 hours. Glucose level, total protein, bactericidal activity, natural agglutination, phenoloxidase, respiratory burst and superoxide dismutase activities in the hemolymph and the shrimp survival rate (SR) were analized before and after the stress tests. Although all examined parameters remained the same between groups before the stress test, significant differences were found among them after the stress test. Micro bubble aeration obtained best survival rate, bactericidal activity, and superoxide dismutase activities in all groups. No significant effect was resulted by the dietary probiotic treatments, but the interaction with aeration treatments revealed the importance to compensate shrimp survival rate when micro bubble aeration is unavailable. It is therefore concluded that micro bubble aeration improves the resistance of whiteleg shrimp postlarvae againt salinity stress, and probiotic BALSS rescues the shrimp populations from salinity stress mortalities when micro bubble is unavailable.
