| Penulis/Author |
IKA PRATIWI (1); BETTY RAHMAWATI (2); Thoriq Teja Samudra (3); Ir. Yano Surya Pradana, S.T., M.Eng., IPM., ASEAN Eng., ACPE., APEC Eng. (4); Prof. Dr.Eng. Ir. Arief Budiman, M.S., IPU. (5); Dr. Eko Agus Suyono, S.Si., M.App.Sc. (6) |
| Abstrak/Abstract |
Biomass produced by microalgae is one of the main sources for producing biofuel, which highly dependable on light intensity since microalgae is an autotroph organism. In laboratory-scale cultivation, light is using to replace the sun light as the main photon energy for microalgae photosynthesis. Variation of light intensity and time interval is mainly used as to manipulate microalgae biomass. The aim of this research was to know the effective light intensity for obtaining high biomass of mixed culture of Glagah and Arthrospira maxima. The mixed culture was cultured in 10 days by using Light Emitting Diode (LED) at 3000, 5000, and 7000 Lux. Thoriq Eko Arif Medium (TEAM) was used as the culture medium. The number of cells and lipid content was measured using Hemocytometer and Bligh and Dyer method, respectively. Then the lipid content per cell were measured based on the cells number and lipid content. The results showed that the highest cell density and dry weight was obtained by using light intensity of 7000 Lux were 385×104 cell.mL−1 on the fifth day and 1.65 mg.mL−1 on the seventh day. The highest concentration of A chlorophyll was resulted by using light intensity of 5000 Lux which was 3.18 μg.gfw−1. Meanwhile the highest B chlorophyll was resulted by using light intensity of 7000 Lux which was 1.41 μg.gfw−1. The intensity light of 5000 Lux was known as to have the highest lipid content there was 0.36 mg.mL−1. The intensity of light of 7000 lux is the most effective way to increase the number of mixed culture cells and lipid content of the Glagah and A. maxima consortium. |
