ACADSTAFF UGM
| Judul/Title | The effect of Kappaphycus alvarezii active fraction on oxidative stress and inflammation in streptozotocin and nicotinamide induced diabetic rats. |
| Penulis/Author | EVY YULIANTI (1); Prof. Dr. Dra. Sunarti, M.Kes. (2); Prof. Dr. Mae Sri Hartati Wahyuningsih, Apt., M.Si. (3) |
| Tanggal/Date | 13 2022 |
| Kata Kunci/Keyword | |
| Abstrak/Abstract | Background.High glucose increase the glycation process which leads to oxidative stress and inflammation, that can cause complications in diabetes. Several medicinal plants have been studied to prevent diabetes and its complications. One of them is Kappaphycus alvarezii, an algae that known has antidiabetic abilities. This study aimed to examine the effect of K. alvarezii active fractions on plasma hydrogen peroxide (H2O2) and tumor necrosis factor α (TNFα) levels, renal NADPH oxidase 4 (NOX4) and nuclear factor κ B (NFκB) gene expressions. Methods. Active fractions were obtained from bioassay-guided fractionation with antiglycation ability. In vivo study was performed in twenty Wistar male rats. The level of H2O2 was measured using H2O2 Assay Kit, the Optical Density value measured using spectrophotometer at a wavelength of 405 nm. Plasma TNFα level was measured using ELISA. NOX4 renal NFκB gene expression was analyzed using qPCR. Results. Active fractions significantly reduced plasma H2O2 but not TNFα levels. Furthermore, renal NOX4 gene expression was lower in the diabetic rat group treated with active fractions compared to the untreated group but not NFκB gene expression. Conclusions. K. alvarezii active fractions has an activity to reduce plasma H2O2 as well as renal NOX4 gene expression. Therefore, this fractions could be developed as a potential candidate for diabetes treatment through oxidative stress mechanisms. |
| Rumpun Ilmu | Ilmu Kedokteran Dasar & Biomedis |
| Bahasa Asli/Original Language | English |
| Level | Internasional |
| Status |