Karya
Judul/Title The Effect of Glutathione Addition in Diluent Semen on Ram Spermatozoa Quality
Penulis/Author MUTHIAH SYAFITRI (1); TEGUH ARI PRABOWO (2); PRADITA IUSTITIA S (3); Prof. Dr. Ir. Lies Mira Yusiati, SU., IPU., ASEAN Eng (4); Dr. Ir. Sigit Bintara, M.Si., IPU., ASEAN Eng. (5); Prof. Ir. Diah Tri Widayati, MP., Ph.D., IPM (6)
Tanggal/Date 24 2022
Kata Kunci/Keyword
Abstrak/Abstract Cryopreservation of ram sperm diminishes sperm motility and annihilates sperm membrane integrity due to free radicals developed by reactive oxygen species (ROS). Moreover, it provokes cryodamage and deteriorates sperm quality. This study investigated the effect of glutathione addition as an antioxidant agent on the quality of local ram spermatozoa. The impacts of glutathione added in five different concentrations (control, 0.5 mM, 1 mM, and 1.5 mM treatment P0-P4) as the extender used to dilute the semen of two rams using andromed diluents was examined. This research examined through several factors, including the quality of fresh sperm, sperm motility, and membrane integrity after chiller. The data in this research was assessed using one-way ANOVA to determine the effect of glutathione addition between the control and treatment groups. The outcomes demonstrated that adding glutathione significantly (p0.05) enhanced motility and membrane integrity in cold storage for 48 hours. In comparison with the control, the P1 concentration generated the best results in terms of ensuring the integrity of ram spermatozoa after processing. Glutathione concentration of 0.1mM (P1) showed the best motility and membrane integrity at 0 hours and 48 hours cold storage (82.50 ± 0.92 versus 65.35 ± 1.80% and 87.50 ± 0.50 versus 68.75 ± 1, 83%. In conclusion, the addition of glutathione affected the quality of local sheep spermatozoa after cooling. We obtain the best results of membrane motility and integrity at the glutathione level of 0.1 nM.
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1Muthiah Syafitri_2022_The Effect of Glutathione Addition_ISTAP 2021_251-255.pdf[PAK] Full Dokumen
2Cek similarity.pdfCek Similarity
3Artikel The effect of Glutathione.pdfArtikel dan Sertifikat/Bukti Kehadiran/Pasport (jika tidak ada sertifikat)
406 Surat Keterangan Publikasi prosiding The Effect of Glutathione.pdfDokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)