| Abstrak/Abstract |
Sambiloto herb (Andrographis paniculata (Burm. f.) Nees.) and pegagan herb (Centella asiatica (L.)
Urban) are excellent medicinal plants which are being developed as traditional for antidiabetic. The research
aims to see the effect of combination from purified extract of sambiloto herb and pegagan herb of
translocation of GLUT-4 protein in model type 2 diabetes mellitus rats-insulin resistance compared with the
use of each purified extract. Test preparations were divided into 8 groups include three from combination
purified extract of sambiloto herb and pegagan herb: I (912,1: 300), II (651,5: 500), III (390,9: 700) in mg/kg
BW, IV (purified extract of sambiloto herb 1303 mg/kg BW), V (purified extract of pegagan herb 1000 mg/kg
BW), VI (positive control metformin 45 mg/kg BW), VII (negative control CMC-Na 0.5%) and VIII (normal
control). Parameters measured semi quantitative data translocation of GLUT-4 protein in cells muscles of the
thigh (soleus muscle) rats using Immunohistochemistry methods. Test animals are of type 2 diabetes mellitus
insulin-resistance was induced by fructose (1,8 g/kg BW) and fat-rich food (95% pellet, 5% egg yolks and 5
mL/ 200 g BW lard) for 70 days. Determination of insulin resistance used 3 parameters such as blood glucose
levels, lipid levels (triglycerides, LDL, cholesterol, and HDL), and hypoglikemic tests with glibenklamid
compared to normal controls animals. Insulin resistance test results using statistical analysis Independent
Samples t-Test showed significant differences (p< 0.05) between normal rats with fat-fructose rats at day-50
and -70. This indicate fat-fructose rats indicated type 2 DM insulin resistance. Group of combination I
(combination purified extract of sambiloto herb and pegagan herb (912,1: 300 mg/kg BB) showed effect of
translocation of GLUT-4 protein was better than each purified extract with significantly p<0.05 with oneway
ANOVA.
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