| Abstrak/Abstract |
Diabetes mellitus (DM) has causes chronic endocrine metabolic disorders that can increase the incidence of infertility. Excessive production of reactive oxygen species (ROS) can trigger oxidative stress reactions and reduce antioxidant content. The increase of ROS can have an impact on the endoplasmic reticulum (ER) stress conditions and inhibit the steroidogenesis of the testes. The objective of the current study is to determine the effect of chlorogenic acid (CGA) as an antioxidant in reducing ER stress condition (mRNA expressions of GRP78, XBP1s), and to increase steroidogenesis (mRNA expressions of 3beta-HSD and 17beta-HSD) in the testis of DM rats. A total of 24 rats were randomly divided into six groups, namely: control group, DM1.5 months, DM2 months, DM group were treated with CGA with three different doses (12.5 mg/kgBW, 25 mg/kgBW, and 50 mg/kgBW), respectively. Testicular tissue was isolated for examination of GRP78, XBP1s, 3beta-HSD, and 17beta-HSD mRNA expressions using RT-PCR. In the CGA1 group, GRP78 mRNA expression level was lower than in the DM2 group and was statistically different (p=0.021). XBP1s mRNA expression in the CGA1 group was lower and significantly different when compared to the DM2 group (p=0.018). The mRNA expression of 3beta-HSD in the CGA1 group was higher than that in the DM1.5 and DM2 groups, which were statistically significant with p=0.000 and p=0,008. The comparison of 17beta-HSD mRNA expression in the CGA1 group was higher than the DM1,5 and DM2 groups with p=0.013 and p=0.012. Administration of CGA at a dose of 12.5 mg/kgBW can reduce ER stress conditions and increase testicular steroidogenesis in DM rat models. These results were seen based on low GRP78 and XBP1s mRNA expressions, and high 3beta-HSD and 17beta-HSD mRNA expressions after CGA administration. |
