| Penulis/Author |
Prof. drg. Titik Ismiyati, M.S., Sp.Pros.(K). (1) ; Dr. drg. Ananto Ali Alhasyimi, MD.Sc., Sp.Ort (2); Prof. Dr. drg. Widowati Siswomihardjo, M.S. (3); Prof. drg. Supriatno, M.Kes., MDsc., Ph.D. (4) |
| Abstrak/Abstract |
This study investigates the effect of chitosan and acrylate acid addition in the acrylic resin as
denture material toward the number of fibroblasts and inflammatory cells including macrophages,
lymphocytes, and plasma cells.
This study is an experimental laboratory research. Nine Wistar rats (Rattus Novergicus) were
divided into 3 groups (n= 3). Control group receiving treatment without mixture of acrylic resin (CO),
group 2 acrylic resin mixture with chitosan and 1% acrylate acid (ARC1), while group 3 acrylic resin
mixture with chitosan and acrylate acid 2% (ARC2). The acrylic resin plate is formed in 4 mm-sized
discs with 2 mm thickness. Resin samples were implanted into subcutaneous dorsal-area of Wistar
rats, then stitched. Microscopic evaluation conducted on day 7 post-implantation. Histological
examination combined hematoxylin eosin staining and 400x magnification light microscope.
Fibroblasts, macrophages, lymphocytes and plasma cells were counted using the Image J program.
Data were analyzed using one-way ANOVA and followed by LSD test with 95% level of
significance.
Results revealed that fibroblasts, macrophages, lymphocytes and plasma cells count in the CO
group were higher than the ARC1 and ARC2 groups. Greater concentration of chitosan and
acrylate acid in the acrylic resin decreased the mean of fibroblasts, macrophages, lymphocyte and
plasma cells. One-way ANOVA test presented significant differences in the fibroblasts,
macrophages, lymphocytes and plasma cells count of the control and treatment groups (p<0.05).
The LSD test indicated significant difference between CO and ARC2 group.
We concluded that the combination of acrylic resin with chitosan and 2% acrylate acid
concentration is properly perceived by rat’s body tissue. |
