| Abstrak/Abstract |
Ethanolic extract and brazilein-containing fraction of Caesalpinia sappan L., has been reported to inhibit cell proliferation on T47D (estrogen receptor positive, luminal A subtype model). The luminal A subtype is the most prevalent subtype of breast cancer in Indonesian females. In this study, we explored the activity of the reduced form of brazilein, brazilin, on T47D cells proliferation and the mechanism that involved. The cytotoxicity activity of brazilin was observed using the MTT assay. The cell cycle profile was analyzed by using flow cytometry, and the cells senescent was observed using S-A-?-galactosidase assay. The results showed that brazilin was cytotoxic to T47D with an IC50 value of 50?M (14.3?g/mL). Cell cycle profile showed that after treated with brazilin, the cells were accumulated at the G2/M phase. Furthermore, cells treated with a combination of brazilin and doxorubicin were accumulated at the G2/M and sub G1 phase. Cells accumulation at sub G1 phase indicates that the cells underwent apoptosis. Our data of S-A-?- galactosidase assay showed that cells treated with 1?4IC50, 1?2IC50, and IC50 brazilin had lower senescent cells than the untreated cells. The morphology of cells treated with IC50 (50?M) brazilin was changed. The cells shape became rounded, cells were shrink and detached from the well plate, indicating that cells may undergo apoptosis. These results suggested that brazilin cytotoxic towards T47D cells, decreased cell senescence, and may induce apoptosis. Therefore, we believe that brazilin is potential to be further examined for its mechanism of action in inhibiting the proliferation of T47D cells.? |
