Immunomodulatory Activity of Combination of Phyllanthus niruri Linn, Typhonium flagelliforme (Lodd.) Blume, and Piper crocatum on Macrophage Phagocytosis In Vitro
Penulis/Author
Reynelda Juliani Sagala (1); drh. Retno Murwanti, M.P., Ph.D. (2); Dr. Andayana Puspitasari Gani, S.Si., M.Si. (3); Dr. Agustinus Yuswanto, SU., Apt. (4)
Tanggal/Date
1 2020
Kata Kunci/Keyword
Abstrak/Abstract
Research on the activity of Phyllanthus niruriLinn, Typhonium flagelliforme(Lodd.) Blume and Piper crocatumhave been conducted and showed various immunomodulatory activity. This study aims to investigate the immunomodulatory activity of the combination of the ethanolic extracts of Phyllanthus niruriLinn, Typhonium flagelliforme (Lodd.) Blume, and Piper crocatum by determining its macrophages phagocytic index and macrophages phagocytic capacity. Therefore, such a combination could be an alternative drug to increase immune response. In this study, the extraction procedure was carried out through maceration by using an ethanolic solvent. Combinations of herbs ethanol extract were varied in four groups of combination, at three different concentrations of 1 ?g/ml, 10 ?g/ml, and 100 ?g/ml for each group. Macrophages were isolated from the peritoneum cavityof male mice (Mus musculus),and its phagocytic activity was quantified through the Leijh method(1986). The phagocytic index and phagocytic capacity of macrophages were determined by using latex beads as a trigger of phagocytosis and compared with negative controlsof media, DMSO, and four groups of ethanolic extract combinations in different concentrations. The results indicate that all of combination group ethanol extract with a concentration of 10 ?g/ml was significantly (p<0.05) optimum activated phagocytic index. Therefore the combination of Phyllanthus niruriLinn, Piper crocatum, and Thyphonium flagelliforme(Lodd.) Blume ethanolic extract might be prospective to increase nonspecific immune response
Rumpun Ilmu
Farmakologi dan Farmasi Klinik
Bahasa Asli/Original Language
English
Level
Nasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Immunomodulatory Activity of Combination of Phyllanthus niruri Linn, Typhonium flagelliforme (Lodd_) Blume, and Piper crocatum on Macrophage Phagocytosis In Vitro.pdf
[PAK] Cek Similarity
2
Sertifikat Reakreditasi 2020.pdf
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3
Surat Pernyataan - Immunomodulatory Activity of Combination of Phyllanthus niruri Linn, Typhonium flagelliforme (Lodd_) Blume, and Piper crocatum on Macrophage Phagocytosis In Vitro.pdf
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
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EC-Reynalda.pdf
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)