The Combination of Carbonate Hydroxyapatite and Human Beta-Defensin 3 to Enhance Collagen Fibre Density in Periodontitis Sprague Dawley Rats
Penulis/Author
IKA ANDRIANI (1); Prof. Dr. apt. Edy Meiyanto, M.Si. (2); drg. Suryono, S.H., M.M., Ph.D. (3); Prof. drg. Ika Dewi Ana, M.Kes., Ph.D. (4)
Tanggal/Date
2020
Kata Kunci/Keyword
Abstrak/Abstract
Background: Carbonate hydroxyapatite (CHA) is used as a scaffold to repair bone resorption. Alveolar bone resorption in periodontitis
caused by an infection requires the presence of an antibacterial to support bone regeneration. Human ?-defensin 3 (HBD3) is an
antimicrobial peptide. The local application of the HBD3 antimicrobial is beneficial to inhibiting drug resistance and protecting tissue
regeneration against invasive bacteria. Purpose: This study aims to investigate the effect of the administration of a combination of CHA
with HBD3 on the collagen density of periodontitis rats (Sprague Dawley). Methods: This study was a true experimental study with a
post-test control group design. Thirty-two Sprague Dawley animal models were randomly blind selected and placed under anaesthetic,
then a 2-mm silk ligature was attached as a ligation to the mandibular incisors for 14 days in order to generate periodontitis. The
study subjects were divided into two groups, the group with CHA and CHA-loaded HBD3 (CHA + HBD3) implantation. On days 7,
14, 21 and 28, four rats were taken randomly from each group for decapitation, followed by histological processing and examination
with trichrome Mallory staining. The data was analysed using the Kruskal–Wallis test (p<0.05). Results: An increase in collagen
density during the healing process was found. There was a significant difference between CHA and CHA+HBD (p=0.004 and p=0.008;
p<0.05) in collagen density between the groups. Conclusion: The combin
