The Capability of Gastrointestinal-origin Lactobacillus casei Strains to Alleviate Hyperglycemia in Rats (Rattus norvegicus): an in vivo Study
Penulis/Author
Prof. Widodo, S.P., M.Sc. Ph.D. (1); PRADIPTA AYU HARSITA (2)
Tanggal/Date
2017
Kata Kunci/Keyword
Abstrak/Abstract
The objective of this study was to evaluate the ability of gastrointestinal-origin probiotics strains in alleviating hyperglycemia in rats (Rattus norvegicus) in vivo. Two strains of Lactic Acid Bacteria consists of Lactobacillus casei strain AP and AG were previously isolated and identified from faeces of Indonesian infants who consumed breast milk as the only source of diet. In vitro evaluation those two strains showed their potential as probiotic based on their capability to grow on media pH 2.0, with 1.5% concentration of bile salts, the ability to attach on gastric mucin in vitro, and their ability to inhibit the growth of pathogen. Evaluation on the ability to use prebiotic inulin as carbon source showed that both strains had the ability to degrade inulin as a prebiotic. Evaluation of probiotics on their capability to synthesize conjugated linoleic acid from free linoleic acid showed that only L. casei strain AP was able to convert more than 60% of free linoleic acid to conjugated linoleic acid in the media. Further studies showed that L.casei strain AP had the ability to alleviate hyperglycemia in rats (Rattus norvegicus). The capability of L. casei strain AP in reducing hyperglycemia was comparable with that of metformin (anti-hyperglycemia drug) provided orally at level 45 mg/kg of body weight.
