Karya
Judul/Title The axenic treatments for Kappaphycus alvarezii (Rhodophyta) seedling in laboratory culture
Penulis/Author Dr. Ratih Ida Adharini, S.Pi., M.Si. (1); Prof. Suadi, S.Pi., M.Agr.Sc., Ph.D (2); Anes Dwi Jayanti, S.Pi., M.Agr. (3); Dr. Eko Agus Suyono, S.Si., M.App.Sc. (4)
Tanggal/Date 2018
Kata Kunci/Keyword
Abstrak/Abstract Obtaining an algae axenic culture in the culture medium are challenging. This study aims to determine the effectiveness of the application of several methods to reduce bacterial contamination in culture medium. The study was conducted using a complete randomized design in 3 stages, stage 1 using iodine immersion method with 4 treatments, stage 2 using immersion of antibiotics mixture with 3 different concentrations, stage 3 using mixture of antibiotic and medium with 3 concentration. The results showed that in stage 1, the lowest bacteria density was in treatment 4 (15 % betadine with 60') (675.27 idvcenterdotmm-2), stage 2 showed that treatment 1 (50 mgcenterdotL-1) had the lowest bacteria density (265.62 idvcenterdotmm-2). Stage 3 showed that treatment 3 (10 mgcenterdotL-1) had the lowest density of bacteria (24.78 idvcenterdotmm-2). Based on the ANOVA test, stage 1 has no significant difference (> 0.05), in stage 2 there was a significant difference with treatment 1 was the best treatment, in stage 3 there was a significant difference and treatment 4 was the best result. Iodine 15 % with 60' immersion time; immersion with 50 mgcenterdotL-1 antibiotic, and mixture of medium with 10 mgcenterdotL-1 antibiotic gave the best results in reducing bacterial contamination.
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1Full dokumen_The axenic treatments.pdf[PAK] Full Dokumen
2similarity The axenic treatments for Kappaphycus alvarezii (Rhodophyta) seedling in laboratory culture.pdfCek Similarity
3Full_Bukti Koresp_the axenic treatments.pdf[PAK] Bukti Korespondensi Penulis