| Abstrak/Abstract |
Cinachyrella sp. marine sponge produce many kinds of secondary metabolites. The purpose of this study was to examine the cytotoxic and anti-proliferative activity of marine sponge Cinachyrella sp. The sponge was extracted with 96 % ethanol. Ethanol extract cytotoxicity assay were performed with MTT method (Microculture Tetrazolium) against to cell lines of HeLa, T47D, WiDr and Vero. The results show that of the ethanol extract were only toxic to cell lines of HeLa IC50 897.809 ?g/mL but did not toxic to cell lines of T47D, WiDr and normal cell lines of Vero. Fractionation of the ethanol extract was conducted by a Vacuum Column Chromatography (VCC) and have 4 fraction that were F1, F2, F3 and F4. Cytotoxicity and cell proliferation inhibitory were tested of fraction F1, F2, F3 and F4 against cell lines of T47D. The values IC50 of F1; F2; F3 and F4 against cell lines of T47D were 82.744; 163.679; 66.522 and 333.026 ?g/mL and fraction F3 concentration 31.5 ?g/mL inhibits cell proliferation cell lines of T47D at 48 hours of incubation.
Keywords? Cinachyrella sp., MTT, Cell Lines HeLa, T47D, WiDr and Vero.
Abstrak? Spons laut Cinachyrella sp. menghasilkan banyak metabolit sekunder. Tujuan penelitian ini mengkaji sitotoksisik dan antiproliferasi spons laut Cinachyrella sp. Spons diekstraksi dengan etanol 96%. Uji sitotoksisitas ekstrak ethanol dilakukan dengan Metode MTT (Microculture Tetrazolium) terhadap sel HeLa, T47D, WiDr dan Vero. Ekstrak ethanol menunjukkan sitotoksik terhadap sel HeLa dengan nilai IC50 897,809 ?g/mL, tetapi tidak toksik terhadap sel T47D, WiDr dan Vero. Ekstrak ethanol difraksionasi dengan metode kromatografi kolom vakum (KKCV). Hasil fraksionasi diperoleh 4 fraksi F1, F2, F3 dan F4. Semua fraksi dilakukan uji sitotoksik dan anti-proliferasi terhadap sel T47D. Fraksi F1, F2, F3 dan F4 menunjukkan sitotoksik terhadap sel T47D dengan nilai IC50 82,744; 163,679; 66,522 dan 333,026 ?g/mL dan fraksi F3 konsentrasi 31,5 ?g/mL menghambat proliferasi sel T47D pada inkubasi 24 jam.
Keywords: Cinachyrella sp., MTT, HeLa, T47D, WiDr, Vero.
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