| Abstrak/Abstract |
Abstract. Organ for the biosynthesis of secondary metabolite is not always a place for its
biosynthesis, even the same compound was synthesized in the different organs in different
plants. Neolignan is a secondary metabolite, known as an imunostimulant, synthesized through
shikimic acid pathway with the important precursor is chorismic acid. The compound was
known to be accumulated in the roots, stems and leaves of Piper regnellii with the
concentration varies depending on the type of neolignan. It has been investigated the
accumulation of two compound neolignans (Pc-1 and Pc-2) isolated from the methanol extract
of red betel leaf (Piper crocatum Ruiz & Pav.) in the leaves, stems, and flowers of red betel.
Chromatographic methods used was Gas Chromatography-Mass Spectrometry (GC-MS).
Chromatogram of GC-MS showed that the Pc-1 with purity of 100%, m/z 460.3 could be
detected at the minute of 29.986, while the Pc-2 with purity of 96.681%, m/z 418.3 was
detected at the minute of 29.495. The research was then continued to investigate the existence
and accumulation of both compounds in leaves, stems, and flowers of red betel. The GC-MS
chromatogram shows that Pc-1 and Pc-2 could be detected in the leaves, stem and flower with
various concentration among plant organs. Moreover, leaves contained the highest
concentration of Pc-1 and Pc-2 compared to other plant organs. |
