ACADSTAFF UGM
| Judul/Title | SYNTESIS AND ANTIBACTERIAL ACTIVITY OF DIBENZYLIDENE-CYCLOHEXANONE |
| Penulis/Author | Prof. Dr. Ritmaleni,S.Si. (1) ; ANNISA NURBAITI AYU HASTUTITAMA (2); INDRIA PERSITTAMAIA (3); TRIBUANA RESTIWARDANI (4); AHMAD EKO PURWANTO (5); A. Eksakta (6); MUHAMMAD SYAUQI A (7); AHMAD EKO PURWANTO (8); Prof. Dr. Puji Astuti, S.Si., M.Sc., Apt. (9); Prof. Dr. apt. Sardjiman, M.S. (10) |
| Tanggal/Date | 2021 |
| Kata Kunci/Keyword | |
| Abstrak/Abstract | Curcumin is as one of the source of compounds for the discovery of new antibacterial agents. Some curcumin analogs have been studied for their antibacterial activities. Some analogs were more active compared to curcumin. This kind of curcumin analogs has a dibenzylidene-cyclic ketone structures. The aim of this study is to synthesize and screen the dibenzylidene-cyclohexanone, as antibacterial agent. Dibenzylidene-cyclohexanone was synthesized by reacting aromatic aldehydes with cyclohexanone in acidic condition through the carbonyl condensation reaction. The reaction was carried out at room temperature, monitored by TLC and the purification process was by recrystallization. The structure was elucidated by using the spectroscopic methods (IR, HNMR, CNMR and MS). The antibacterial activity was tested by using microdillution method against K. pneumonia, E. coli, S. aureus, B. subtilis and E. faecalis. The isolated products were obtained as pure curcumin analogs with moderate to high yield. The pure products were also confirmed as curcumin analog, dibenzylidene-cyclohexanones. The antibacterial assay showed that 2,6-bis-(3’-hydroxybenzylidene)-cyclohexanone gave MIC and MBC at 50 µg/mL in E. coli, S. aureus, and E. faecalis. Moreover, it showed that the percentage inhibition of B. subtilis was around 54 % at concentration of 25 µg/mL. Among all the compounds, A146 gave a better activity against all that bacteria while none gave activity against K. pneumonia. The docking study of the activity was also been studied. It was found that A111 was compound with the highest yield, A146 was the most potent one and A102 was the most potent one according to docking study due to its smallest score docking among tested compounds. |
| Rumpun Ilmu | Analisis Farmasi dan Kimia Medisinal |
| Bahasa Asli/Original Language | English |
| Level | Internasional |
| Status |