| Abstrak/Abstract |
Background and Aim: Kefir, a natural probiotic containing bacteria and yeast, is a fermented milk product, whereas
glucomannan from porang tuber (Amorphophallus oncophyllus) is prebiotic in vivo. Simvastatin is a potent lipid-lowering
statin that can be utilized for pharmacological therapy in obesity. This study aimed to determine the effect of goat milk kefir
supplemented with porang glucomannan (synbiotic kefir) and goat milk kefir without glucomannan (probiotic kefir) on
blood glucose, hemoglobin A1c (HbA1c), free fatty acids (FFAs), tumor necrosis factor-alpha (TNF-α), gene expression
of peroxisome proliferator-activated receptor gamma (PPARγ), and insulin-producing cells in rats fed a high-fat and highfructose (HFHF) diet.
Materials and Methods: Male Sprague-Dawley rats were divided into five dietary groups: (1) Normal control, (2) rats fed
HFHF, (3) rats fed HFHF+probiotic kefir, (4) rats fed HFHF+synbiotic kefir, and (5) rats fed HFHF+simvastatin. All of
these treatments were administered for 4 weeks.
Results: There were no significant differences in plasma glucose levels in HFHF diet-fed rats before and after treatment.
However, plasma HbA1c and TNF-α decreased, and FFAs were inhibited in rats after treatment with synbiotic kefir.
Synbiotic kefir decreased the gene expression of PPARγ2 in HFHF diet-fed rats but did not affect the total number of islets
of Langerhans and insulin-producing cells.
Conclusion: Synbiotic kefir improved the health of rats fed an HFHF diet by decreasing HbA1c, TNF-α, and PPARγ2 gene
expression and preventing an increase in FFAs. |
